PROFORMA
Laboratory
Recognition under Environment (Protection) Act, 1986
(To be filled in by
existing laboratories interested to be considered for recognition as
Environmental Laboratories under the Environment Protection Act, 1986)
1. General
(i) Name of the Laboratory :__________________________________
___________________________________
(ii) Year of
Establishment :__________________________________
(iii) Name of the
Organisation :__________________________________
(iv) Address:
a) Postal :__________________________________
___________________________________
___________________________________
b) Telephone :__________________________________
c) Telegram :__________________________________
d) Telex/fax :__________________________________
(v) Objectives
and scope of the Organisation: *
*(Please indicate, among others, whether it includes
specialized testing, measurement, services)
(vi) Head of the Organisation:
a) Name :__________________________________
b) Designation :__________________________________
c) Address :__________________________________
___________________________________
___________________________________
d) Telephone No. :_________________
Fax No. : _________
(vii) Laboratory Incharge, if different than (vii) above:
a) Name :__________________________________
b) Designation :__________________________________
c) Address :__________________________________
___________________________________
___________________________________
d) Telephone No. :_________________
Fax No. : _________
2. Infrastructure and details of
Laboratory: (please enclose brief layout plan map of laboratory)
(i) Area of specialisation pertaining
to Environment:
a) ________________________________________________________
b) ________________________________________________________
c) ________________________________________________________
d) ________________________________________________________
e) ________________________________________________________
(ii) Type of
tests which are offered to be conducted:
(Please enclose separate sheet, if
space is inadequate)
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S.No. |
Nature of tests / analysis performed |
Minimum required time for the test / analysis |
Charges |
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(iii) Which of the following type of analytical
tests are being carried out in the laboratory:
a) Physical
b) Chemical
c) Radioactive
d) Microbiological
e) Toxicological
f) Any
other (please specify)
(iv) a) Mark the parameters given in Appendix'
A ' which can be analysed in the laboratory
b) Mark
the equipment given in Appendix '8' which are available in the laboratory
c) Mark
the Instruments given in Appendix 'C' which are available in the
laboratory
d) Mark
the methodology employed for analysis in Appendix 'D'
e) Mark
the Air Quality Parameters, which can be analysed in
the laboratory in Appendix 'E'
(v)
Name, designation and qualifications of
staff/officers (with expertise in environmental analysis/testing):
(please enclose separate sheet, if space is inadequate)
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S.No. |
Name |
Qualification |
Experience |
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Note: Please indicate the name/s of personnel
(maximum three) to be considered for nomination as Govt. Analysts. Brief
bio-data of these persons should be enclosed.
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S.No. |
Name |
Qualification |
Experience |
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Signature (Head of organization) (Head of laboratory)
Full name :_____________________ Full name:__________________
(In capital letters) (In capital letters)
APPENDIX
'A'
List of
Parameters to be Analysed
(a) Physical
Tests: [Please mark Yes ( √) / No (x)]
1. Colour
2. Conductivity
3. pH
4. Suspended
solids
5. Settleable solids
6. Sludge
Volume Index
7. Total solids
8. Temperature
9. Turbidity
10. Velocity of
flow
(b) Chemical
Tests
1. Acidity
2. Alkalinity
3. Aluminium
4. Arnmonical Nitrogen
5. Arsenic
6. Barium
7. Beryllium
8. Boron
9. Bromide
10. Carbon
dioxide
11. Cadmium
12. Chemical
Oxygen Demand (COD)
13. Bio-chemical
Oxygen Demand (BOD)
14. Chloride
15. Residual
Chlorine
16. Calcium (Titrimetric)
17. Chromium (Colorimetric)
18. Copper
19. Cyanide
20. Dissolved
Oxygen
21. Fluoride
(Colorimetric without distillation)
22. Hardness (Titrimetric )
23. Iodine
24. Iron
(Colorimetric)
25. Lead
(Colorimetric)
26. Manganese
27. Magnesium
28. Mercury
29. Nickel
30. Nitrite
-Nitrogen
31. Nitrate
-Nitrogen
32. Oil and
Grease (Simple extraction)
33. Pesticides
and Insecticides
34. Phosphate
35. Phenols
36. Potassium
37. Selenium
38. Silica
39. Silver
40. Sodium
41. Strontium
42. Sulfite
43. Sulphate (Turbidimetric)
44. Tannin and
Lignin
45. Tin
46. Urea Nitrogen
47. Zinc
(Colorimetric)
48. Any other ( Please specify )
(c) Radioactive
Tests
1. Gross Alpha
and Gross Beta radioactivity
(d) Microbiological
Tests
1. Faecal Coliform
2. Total Coliform
3. Faecal Streptococci
4. E. Coli
5. Total plate
count
6. Cell Count
(Plankton)
7. Benthos (Mud
organisms)
8. Chlorophyll
(e) Toxicological
Tests
1. Pathways
into the Environment
2. Concentrations:
·
Loss/Persistence
·
Concentrations
·
Human Intake
3. Environmental
Fate Tests:
·
B i odegradation / Biotransforrnation
·
Photodegradation
·
Hydrolysis
·
Sorption
·
Evaporation
·
Oxidation
·
Model Ecosystem studies
4. Environmental
Fate
5. Chemobiokinetics
·
Absorption
·
Distribution
·
Bioconcentration Factor
·
Metabolism
·
Extraction
6. Mammalian
Toxicity
7. Special
Toxicity Studies
·
Biochemical Interactions
·
Carcinogenicity
·
Mutagenicity
·
Neurotoxicity
·
Behaviour
·
Sensitization
·
Interacting Agents
·
Primary Irritation
·
Irnrnunotoxicity
·
Reproduction
·
Teratogenicity
8. Effects on
Organisms in the Environment
·
Aquatic Toxicity
·
Terrestrial Toxicity
APPENDIX 'B'
(LIST OF EQUIPMENTS)
[Note
: Please mark mark Yes (Ö) / No (x)]
1. Autoclave
2. Aquarium for bioassay & aerators
3. Ammonia distillation assembly
4. Arsenic estimation assembly (Gutzeit generator)
5. Balance (one weighing upto 10 mg & other weighing upto
0.01 mg)
6. BOD Incubator
7. Bacteriological Incubator
8. Bottom sampler
9. Centrifuge
10. Colony Counter (Electronic)
11. Colour
Comparator
12. Current Meter
13. COD Digestion Assembly
14. Cyanide distillation Assembly
15. Deep Freezer
16. Depth Sampler
17. Electronic Colony Counter
18. Filtration Pumps (Vaccum)
19. Fluoride distillation assembly
20. Flocculator
(Jar testing apparatus)
21. Flow meter (air, water)
22. Flask shaker
23. Fuming chamber
24. Gas Analysis apparatus
25. Heating Mentle
26. Hot Plate (different sizes)
27. Inoculation chamber
28. Inoculation hood
29. Kjeldahl
Nitrogen Assembly
30. Magnetic Stirrer with hot plate
31. Millipore filtration assembly
32. Muffle furnace
33. Oven (hot air)
34. Refrigerator (big size)
35. Rotary shaker
36. Semi-micro nitrogen assembly
37. Stop watch
38. Thermometer (different ranges)
39. Soxhlet
extraction assembly
40. Vaccum
filtration pump
41. Water current meter
42. Water deionizer
43. Water distillation assembly (nano pure system)
44. Water distillation assembly (ordinary)
45. Water bath
46. Microscope (ordinary)
47. Microscope (Research)
48. Microscope (Planktonic
inverted)
49. Any other (Please specify)
APPENDIX 'C'
LIST OF
INSTRUMENTS
[Note
: Please mark mark Yes (Ö) / No (x)]
1. Analytical Balance
2. Atomic Absorption Spectrophotometer
with the following cathode lamps:
Aluminium
Antimony Arsenic Barium
Boron
Chromium Calcium Cobalt
Copper Iron
Lead Magnesium Manganese Nickel Potassium
Silver Sodium Tin Vanadium
Zinc
3. Binocular Microscope
4. Conductivity Meter
5. Colorimeter
6. Dissection Microscope
7. Dissolved Oxygen Meter
8. Flame Photometer
9. Gas Liquid Chromatograph
10. Mercury Analyzer
11. Micro Analytical Balance
12. Portable Water Analysis Kit (for DO,
Temperature, Conductivitity, Redox
potential etc.)
13. pH Meter with
combined glass-calomel electrode (Portable and Table models)
14. Polarograph
15. Spectrocolorimeter
16. Spectrophotometer (Ultra-Voilet, Visible & Infra-red)
17. Specific Ion Meter with Micro-processor
with the following ion selective electrode:
Ammonia
Iodide Sulphate
Cyanide
Nitrate
Fluoride
Redox
18. Specific Ion Meter (Ordinary)
19. Stereoscopic Microscope
20. Total Organic Carbon Analyzer
21. Turbidity Meter
22. UV -visible Spectrophotometer
23. BOD-Respirometer
24. Any other, please specify or attach the
list.
APPENDIX 'D'
METHODOLOGY
EMPLOYED FOR ANALYSIS
(A) PHYSICAL
PARAMETERS
S.No. PARAMETER METHOD
1. Colour a.
Visible Cornparision Method (only potable waters)
b.
Spectrophotometric Method (All)
2. Conductivity Conductivity Meter
3. pH Value pH
Meter
4. Suspended Solids a. Membrane filter apparatus
(Total
Number (Millipore vaccum filteration apparatus)
filterable) b.
Gooch Crucible
5 Settleable solids Imhoff concentration:
a. By Volume (mg/L)
b.
By Weight (mg/L)
6. Sludge
Volume Index Imhoff
conc. membrane filter apparatus or Gooch
crucible
7. Total
Solids Gravimetric
8. Temperature Thermometer/Thermistor/ Thermophone
9. Turbidity Nephelometric
10. Velocity
of flow a.
Cross-Section-velocity Method
b.
Weirs (Rectangular or V Notch or U-Notch)
c.
Chemical Methods
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(B) CHEMICAL PARAMETERS
S.No. PARAMETER METHOD
1 Acidity a. Electrometric/Potentiometric titration
b.
Color Indicator titration
2 Alkalinity
a. Electrometric/Potentiometric titration
b.
Color Indicator titration
3. Aluminium a.
Atomic Absorption Spectrophotometer
b.
Colorimetric (Eriochrome Cyaniner)
4. Ammonical a. Distillation followed
by colorimetric
Nitrogen method (Nesselerization or phenate)
b.
Distillation followed by titrimetric method
c.
Distillation followed by ion Selective electrode
method
5. Arsenic a. Atomic
Absorption spectrophotometer
b.
Silver Diethyl dithiocarbonate
6. Barium Atomic Absorption
Spectrophotometer
7. Beryllium a. Atomic Absorption
Spectrophotometer
b.
Colorimetric (Aluminion)
8. Boron Colorimetric (Curcumin or Carmine)
9. Bromide Colorimetric (Chloramine -T)
10. Carbon a. Titrimetric
Dioxide b. Nomographic
11. Cadmium a. Atomic Absorption
Spectrophotometer
b.
Colorimetric (Dithiozone)
12. Chemical Oxygen Dichoromate
Reflux
Demand
13. Biochemical a. Five
day BOD at 20 degree celsius
Oxygen Demand b.
Three day BOD at 27 degree celsius
14. Chloride Titrimetric
(Argentometric or Mercuric Nitrate)
15. Chlorine
Residual Titrimetric
16. Calcium a. Atomic Absorption
Spectrophotometer
b.
Titrimetric (EDTA)
17. Chromium a. Atomic Absorption
Spectrophotometer (For Total)
b. Colorimetric (Diphenylcarbazide)
(For hexavalent,
trivalent and total)
18. Copper a. Atomic
Absorption Spectrophotometer
b. Colorimetric (Neocuproin and Bethno-cuproine)
19. Cyanide a. Distillation
followed by Titrimetric
(P-dimethyl-amino-benzalrhodenino indicator)
b.
Distillation followed by Colorimetric
(pyridine -Barbituric acid)
c.
Distillation followed by Cyanide-Selective
Electrode
20. Dissolved
Oxygen Winkler titrimetric-azide modification (Iodometric)
21. Fluoride a. Distillation
followed by Colorimetric
(SPADNS
or Alizarin Red)
b.
Distillation followed by Fluoride selective
electrode
22. Hardness
Total Titrimetric (EDTA)
23 Iodine Colorimetric (Leuce Crystal violet of Catalytic
reduction)
24. Iron a. Atomic
Absorption Spectrophotometer
b.
Colorimetric (Phenanthraline)
25. Lead a. Atomic
Absorption Spectrophotometer
b.
Colorimetric (Dithiozone)
26. Manganese a. Atomic Absorption
Spectrophotometer (Total)
b.
Colorimetric (Persulfate)
27. Magnesium a. Atomic Absorption
Spectrophotometer
b.
By difference (between total hardness and
calcium determined titri-metrically)
28. Mercury a. Flarneless (cold vapour) Atomic
Absorption
(Mercury
Analyzer)
b.
Colorimetric (Dithiozoncer)
29. Nickel a. Atomic
Absorption Spectrophotometer
b. Colorimetric (Heptaxime or Dimethyl-glyoxime)
30. Nitrite
Nitrogen Colorimetric (Diazotization)
31. Nitrate a. Colorimetric (Chromotropic Acid)
Nitrogen b. Cadmium reduction
followed by Colorimetric for
Nitrite
(Diazotization)
c. Ultra-violet Spectrophotometric
(For unpolluted
water only)
32. Oil
and Grease a. Gravimetric
(Simple extraction)
b.
Soxhlet extraction
33. Pesticides
and a. Organochlorine
-Gas Chromatograph
Insecticides b. Organophosphorus
-Gas Chromatograph
34 Phosphate a.
Orthophosphate-Pretreatment followed by
Colorimetric
(Stannous -chloride or Vanadinrn
molybdate)
b.
Polyphosphate - -do-
c. Total -do
-
35. Phenols a. Distillation
followed by Colorimetric
(4-Aminoantipyrine
or Chloroform extraction)
b.
Gas Liquid Chromatograph
36. Potassium a. Atomic Absorption
Spectrophotometer
b.
Flame photometer
37. Selenium a. Atomic Absorption
spectrophotometer
b.
Distillation followed by colorimetric
(Diaminobenzidine)
38. Silica a. Atomic
Absorption Spectrophotometer
b. Colorimetric (Heteropoly blue
or Molybdosilicate)
39. Siliver a.
Atomic Absorption Spectrophotometer
b.
Colorimetric (Dithiozone)
40. Sodium a. Atomic Absorption
Spectrophotometer
b.
Flame photometer
41. Strontium a. Atomic Absorption
Spectrophotometer
b.
Flame photometer
42. Sulphite Titrimetric (Iodometric)
43. Sulphate Turbidimetric
44. Tannin
and Lignin Colorimetric
45. Tin
Atomic
Absorption Spectrophotometer
46. Urea
Nitrogen a. Colorimetric
b.
Titrimetric
47. Zinc a. Atomic
Absorption Spectrophotometer
b.
Colorimetric (Dithiozone or Zincon)
__________________________________________________________________
APPENDIX
'E'
AIR
PARAMETERS
Mark the air quality
parameters given below which can be analysed in your laboratory , {Please mark Yes (Ö), No (x)}. For yes
marked parameters give details such as method of analysis, instrument used for
sampling and analysis, range of sensitivity etc. Enclose separate sheet.
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Parameters |
Ambient air/Fugitive
Emissions |
Method |
Stack gases/Source
emission |
Method |
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Nox |
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SPM |
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SO2 |
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NO |
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CO |
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Hydro Carbon |
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NH3 |
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O3 |
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Velocity |
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Flow |
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Acid mist |
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SO3 |
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HF |
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Total Fluoride |
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Particulate Fluoride |
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Gaseous Fluoride |
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AIR PARAMETERS
(Contd.)
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Micro meteorological |
Yes/No |
Method |
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1. Wind Speed |
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2. Wind Direction |
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3. Temperature |
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4. Mixing depth
Inversion height |
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Vehicular Emission |
Yes/No |
Method |
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1. Smoke |
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2. Carbon monoxide |
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3. Oxides of
Nitrogen |
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4. Hydrocarbon |
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Toxic And Hazardous Gases
Provide a list of
toxic and hazardous gaseous parameters, which could be analysed
in your laboratory:
___________________________________________________________________
___________________________________________________________________
___________________________________________________________________
___________________________________________________________________
___________________________________________________________________
___________________________________________________________________
___________________________________________________________________
___________________________________________________________________
List of
Instruments / Equipments for Air Analysis
Mention
the names of the instruments / equipment which are available in the laboratory
for sampling and analysis of each of the following group of tests. Also mention
sensitivity and range of each instrument / equipment.
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Instrument |
Make |
Range |
Sensitivity |
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1. Ambient Air/Gigitive Emission |
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2. Micro-meteorological |
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3. Source Emission |
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4. Vehicular Emission |
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5.
Toxic and Hazardous Gases
6.
Does the facilities for calibration of
various flow measuring devices (e.g. Rotameter, dry
gas flow meter), Blower of high volume sampler, Pilot tube etc., exist in your
lab. If yes, then please provide the details of calibration system. (Use
separate sheet if required).
Rules of Environment (Protection) Act –
1986
(The Gazette of
Rule 9 Functions of Environmental
Laboratories
The
following shall be the functions of environmental laboratories:
1.
to evolve standardised
methods for sampling and analysis of various types of environmental pollutants
2.
to analyse samples sent by the Central Government or the
officers empowered under Sub-Section (I) of Section II.
3.
to carry out
such investigations as may be directed by the Central Government to lay down
standards for the quality of environment and discharge of environmental
pollutants, to monitor and to enforce the standards laid down.
4.
to send
periodical reports regarding its activities to the Central Government.
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